Description
For Sample This immunoassay kit allows for the in vitro quantitative determination of human PSA concentrations in serum, plasma, cell culture supernates and tissue homogenates.
PRINCIPLE OF THE ASSAY The microtiter plate provided in this kit has been pre-coated with an antibody specific to PSA. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated antibody preparation specific for PSA and incubated. Then substrate solutions are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PSA in the samples is then determined by comparing the O.D. of the samples to the standard curve